人类脐带的与胎盘绒毛的间充质干细胞的生物学特性比较研究
比较脐带的与胎盘绒毛的间充质干细胞的生物学特性，发现二者均来自胎儿组织，成纤维母细胞外观，表达间充质干细胞表面标志CD90、CD73、CD105、CD44,，不表达CD45、 CD11b、CD34，能分化为成骨细胞和脂肪母细胞，抑制外周血单个核细胞针对PHA刺激分泌的干扰素。只是绒毛间充质干细胞的脂滴较大，抑制分泌能力更强。提示绒毛间充质干细胞更有利于治疗自身免疫性疾病。。
延伸：间充质干细胞存在于骨髓、脂肪、滑膜、骨骼、肌肉、肺、肝、胰腺等组织以及羊水、脐带血、脐带胎盘中。其中对供者损伤最小的是脐带血、脐带胎盘组织。而采集方便，分离容易，培养高效的非脐带胎盘组织莫属。韩忠朝率领其团队于2006年4月，在滨海新区组建了世界第一个间充质干细胞库。http://www.amcellgene.com/about-us-root/about-us/honour.html
8. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Jun;20(3):692-6.
[Comparative study of biological characteristics of human umbilical cord and placental chorionic villous mesenchymal stem cells].
[Article in Chinese]
Han ZB, Yang ZX, Chi Y, Wang YW, Wang T, Ji YR, Yang P, Meng L, Han ZC.
Source
Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences, Tianjin, China.
Abstract
Because advantage of tissue origin and proliferation potential, the umbilical cord-derived mesenchymal stem cells (UC-MSC) and placental chorionic villous-derived mesenchymal stem cells (CV-MSC) have clinical application potential, as compared with bone marrow MSC. But whether the differences of biological characteristics exist between UC-MSC and CV-MSC, which deserve to be further explored. This study was purposed to compare the biological characteristics of UC-MSC and CV-MSC. The placental and umbilical cord were cleaned by using the sterile physiological salt, the UC-MSC and CV-MSC were separated by enzyme digestion. Short tandem repeat (STR) analysis was used to detect whether the MSC obtained from fetal tissue. MTT method was used to detect proliferation of MSC. Flow cytometry was applied to analyze cell phenotype. The different differential medium was used to detect their multi-directional differentiation capacity. After the MSC and PHA-stimulated peripheral blood mononuclear cells were co-cultured, the γ-interferon (IFN-γ) levels of the co-culture supernatant were detected using the ELISA. The results showed that these MSC were derived from fetal tissue by STR analysis. They were adherent cells with typical fibroblast morphology. Cells expressed the MSC surface markers CD90, CD73 and CD105 and CD44, not expressed CD45 and of CD11b and CD34.These cells could differentiate into osteoblasts and adipoblasts under culture with different conditioned medium, but in the adipogenic differentiation of CV-MSC, the larger lipid droplets appeared. It is concluded that these cells are obtained MSC. These MSC can inhibit peripheral blood mononuclear cells stimulated by PHA to secrete IFN-γ, and the the CV-MSC have a stronger suppression capacity, which makes the CV-MSC to have a greater advantage in the treatment of autoimmune diseases.
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