扩增晚期内皮祖细胞方法
利用滋养层细胞（人脐静脉内皮细胞和晚期内皮祖细胞）不但能够使晚期内皮祖细胞的克隆数增加20倍，而且晚期内皮祖细胞克隆出现更早。
延伸：内皮祖细胞在心脑血管疾病、外周血管疾病、肿瘤血管形成及创伤愈合等方面均发挥重要作用，并为缺血性疾病的研究治疗提供了新思路。而最新的一项研究证明血管疾病的真凶是干细胞的异化（http://www.nature.com/ncomms/journal/v3/n6/full/ncomms1867.html）。对内皮祖细胞扩增方法及其微环境的研究有助于对血管疾病的了解与治疗。
2. Zhonghua Yi Xue Za Zhi. 2012 Sep 25;92(36):2574-7.
[Method of expansion of late endothelial progenitor cells].
[Article in Chinese]
Wu LH, Song ZX, Liu XH, Li SZ, Han ZC, Uzan G.
Source
Department of Internal Medicine, Chinese Academy of Medical Sciences, Tianjin, China. [email protected]
Abstract
OBJECTIVE:
To validate a novel method of expanding late endothelial progenitor cells (EPC) in vitro.
METHODS:
We cultured mononuclear cells (MNC) from human peripheral blood on the plate with the feeder layer cells, i.e. irradiated late EPC or human umbilical vein endothelial cells. After 21 days, the numbers of late EPC colonies were counted separately. And the surface antigen of late EPC was detected by fluorescence-activated cell sorter (FACS) and their in vitro ability of forming vascular structure examined by matrigel.
RESULTS:
Both colony numbers of late EPC with feeder layer cell culturing were over 20 times than those without (40.0 ± 3.9, 39.3 ± 3.1 vs 2.0 ± 1.3, both P < 0.05). And the former's late EPC colony appeared earlier. The late EPC expressed CD31, CD34, eNOS, Flt-1, P1H12, Sendo, VE cadherin and CD117. And vascular structures were discerned.
CONCLUSIONS:
The method of feeder layer cells may vastly expand the quantity of late EPC. And microenvironment plays an important role in its expansion.
